Research Services
Custom iPSC Generation Packages
Packages include the generation of clonal iPSC lines from a patient sample provided by the customer and a Complete or Essential characterization package. Pricing listed below is for academic institutions only. For commercial pricing, please contact the biomanufacturing center directly.
| Package | Characterization Suite | Price (ACADEMIC) |
| 2 clones | Complete | $13,158 |
| 1 clone | Complete | $11,672 |
| 2 clones | Essential | $8,277 |
| 1 clone | Essential | $7,003 |
Complete Characterization Package includes: Mycoplasma Testing, AP Staining, ICC for Pluripotency Markers, G-Band Karyotype, RT-qPCR for Endogenous Pluripotency gene expression, hPSC Scorecard Assay, EBNA1 gDNA PCR, Cell Line Authentication (STR)
Essential Characterization Package includes: Mycoplasma Testing, AP Staining, ICC for Pluripotent Markers, G-Band Karyotype, Cell Line Authentication (STR)
Source tissues can include fibroblast (skin), fresh whole blood (PBMCs), MSCs, adipose cells, various stromal epithelial cell types, and lymphoblastoid cell lines (LCLs or EBV B-cells). The fresh blood must be collected in CPT tubes and centrifuged according to the manufacturer’s directions. All reprogramming is performed using non-integrating techniques in defined media (serum and KSR-free) and feeder-free conditions (no MEFs). Cost for each patient line includes the expansion and banking of three thoroughly characterized clonal lines under feeder-free conditions. Each cell line will be accompanied by a rigorous Certificate of Analysis to ensure pluripotency, normal karyotype, embryoid body formation and a variety of services related to the characterization of the pluripotent state (see below).
PSC Quality Control and Characterization
The CBC iPSC Core offers a variety of assays to ensure the quality and pluripotency of PSC lines. PSC characterization is included in the custom iPSC generation service or separately as needed.
Service |
Requirements |
Price / iPSC Line (1 clone) Academic |
Price/iPSC Line (1 clone) Commercial |
|
|---|---|---|---|---|
|
Mycoplasma Testing |
Please provide the core with at least 1ml of 2-day-old conditioned media. The core regularly performs mycoplasma testing once a month. Contact the core for the next available mycoplasma testing date. |
$21 |
$34 |
|
|
Mycoplasma Testing (qPCR Method) |
Please provide the core with either 1ml aliquot of supernatant OR 1 cell pellet. |
$135 |
$215 |
|
|
Alkaline Phosphatase (AP) Staining |
Please provide the core with 1 well of 50-60% confluent cells. |
$42 |
$68 |
|
|
Immunocytochemistry (ICC) staining |
Please provide the core with 1 well of good quality cells that are at least 50-60% confluent. |
$84 |
$135 |
|
|
G-Band Karyotype |
Please provide the core with 2 80% confluent wells. |
$530 |
$843 |
|
|
Please provide the core with 1-2 wells of good quality cells that are at lease 80% confluent |
$127 |
$203 |
|
|
Embryoid Body (EB) Formation |
Please provide the core with a 70-80% confluent 6-well plate of cells. |
$424 |
$674 |
|
|
hPSC Scorecard Assay |
Assay is run on undifferentiated iPSCs (Day 0 iPSCs) and spontaneously differentiated EBs. Price is reflected for the pair. Price does not include EB generation. |
$742 |
$1,200 |
|
|
EBNA1 gDNA PCR |
Confirm lack of exogenous plasmid presence. Price is per clone. |
$254 |
$405 |
|
|
Identity Testing via Short Tandem Repeat Analysis (STR) (Unique Sample) |
Please provide the core with 1 cryopreserved vial of cells OR 1 cryopreserved cell pellet. Optional – You may also provide a reference cell line for comparison. |
$190/sample |
$470/sample |
PBMC Isolation And Processing
Service | Requirements | Price/ Sample |
|---|---|---|
Peripheral Blood Mononuclear Cell (PBMC) Isolation and Processing | Fresh whole blood collected in 8ml BD CTP Vacutainer tubes | $397 |
The CBC iPSC Core will isolate peripheral blood mononuclear cells (PBMCs) from freshly collected whole blood. Cells will be cryopreserved at 5,000,000 cells per vial. The iPSC Core recommends collecting a minimum of two (2) 8ml CPT Vacutainer tubes. Please see the references below for more information on the collection of whole blood as well as the shipment of samples to the CBC or contact us.
Biobanking And PSC Thawing
As part of the custom iPSC generation service, the core will expand and bank three fully characterized iPSC clones per cell line. This service is also available to iPSC and ESC lines generated outside of Cedars-Sinai.
Service | Service Description | Requirements | Price/iPSC Line (1 clone) Academic | Price/iPSC Line (1 clone) Commercial | |
|---|---|---|---|---|---|
Cell Line Cryopreservation (5-6 vials) | Guaranteed cryopreservation of high quality, mycoplasma negative cells (6 vials total-feeder free). | Please provide the core with 1 well of starter cells. | $197 | $334 | |
| The core will thaw 1 vial of PSCs into 1 Matrigel coated well of a 6-well plate and maintain cells for 1 week. | Please provide the core with 1 vial of cryopreserved cells. | $63 | $111 | |
| The core will thaw 1 vial of PSCs into 1 Matrigel coated well of a 6-well plate and expand cells into a full 6-well plate over the course of 2 weeks. | Please provide the core with 1 vial of cryopreserved cells. | $212 | $371 | |
PSC Research Grade Biobank (20 vials) | The core will generate a research grade bank of multiple cryopreserved vials of PSCs. | Karyotype will be performed prior to initiating banking and at the time of creating a research grade lot. | Contact Us | Contact Us |