Research Services

Custom iPSC Generation Packages

Packages include the generation of clonal iPSC lines from a patient sample provided by the customer and a Complete or Essential characterization package. Pricing listed below is for academic institutions only. For commercial pricing, please contact the biomanufacturing center directly.

Package	Characterization Suite	Price (ACADEMIC)
2 clones	Complete	$13,158
1 clone	Complete	$11,672
2 clones	Essential	$8,277
1 clone	Essential	$7,003

Complete Characterization Package includes: Mycoplasma Testing, AP Staining, ICC for Pluripotency Markers, G-Band Karyotype, RT-qPCR for Endogenous Pluripotency gene expression, hPSC Scorecard Assay, EBNA1 gDNA PCR, Cell Line Authentication (STR)

Essential Characterization Package includes: Mycoplasma Testing, AP Staining, ICC for Pluripotent Markers, G-Band Karyotype, Cell Line Authentication (STR)

Source tissues can include fibroblast (skin), fresh whole blood (PBMCs), MSCs, adipose cells, various stromal epithelial cell types, and lymphoblastoid cell lines (LCLs or EBV B-cells). The fresh blood must be collected in CPT tubes and centrifuged according to the manufacturer’s directions. All reprogramming is performed using non-integrating techniques in defined media (serum and KSR-free) and feeder-free conditions (no MEFs). Cost for each patient line includes the expansion and banking of three thoroughly characterized clonal lines under feeder-free conditions. Each cell line will be accompanied by a rigorous Certificate of Analysis to ensure pluripotency, normal karyotype, embryoid body formation and a variety of services related to the characterization of the pluripotent state (see below).

PSC Quality Control and Characterization

The CBC iPSC Core offers a variety of assays to ensure the quality and pluripotency of PSC lines. PSC characterization is included in the custom iPSC generation service or separately as needed.

Service

Requirements

Price / iPSC Line (1 clone) Academic

Price/iPSC Line (1 clone) Commercial

Mycoplasma Testing

Please provide the core with at least 1ml of 2-day-old conditioned media. The core regularly performs mycoplasma testing once a month. Contact the core for the next available mycoplasma testing date.

$21

$34

Mycoplasma Testing (qPCR Method)

Please provide the core with either 1ml aliquot of supernatant OR 1 cell pellet.

$135

$215

Alkaline Phosphatase (AP) Staining

Please provide the core with 1 well of 50-60% confluent cells.

$42

$68

Immunocytochemistry (ICC) staining SSEA4 + OCT4 + TRA-1-60 + NANOG + TRA-1-80 + SOX2

Please provide the core with 1 well of good quality cells that are at least 50-60% confluent.

$84

$135

G-Band Karyotype

Please provide the core with 2 80% confluent wells.

$530

$843

RT-qPCR for endogenous/exogenous pluripotency gene expression

Please provide the core with 1-2 wells of good quality cells that are at lease 80% confluent

$127

$203

Embryoid Body (EB) Formation

Please provide the core with a 70-80% confluent 6-well plate of cells.

$424

$674

hPSC Scorecard Assay

Assay is run on undifferentiated iPSCs (Day 0 iPSCs) and spontaneously differentiated EBs. Price is reflected for the pair. Price does not include EB generation.

$742

$1,200

EBNA1 gDNA PCR

Confirm lack of exogenous plasmid presence. Price is per clone.

$254

$405

Identity Testing via Short Tandem Repeat Analysis (STR) (Unique Sample)

Please provide the core with 1 cryopreserved vial of cells OR 1 cryopreserved cell pellet. Optional – You may also provide a reference cell line for comparison.

$190/sample

$470/sample

Biomanufacturing Immunocytochemistry Markers

Biomanufacturing stem-cell characterization

PBMC Isolation And Processing

Service

Requirements

Price/ Sample

Peripheral Blood Mononuclear Cell (PBMC) Isolation and Processing

Fresh whole blood collected in 8ml BD CTP Vacutainer tubes

$397

The CBC iPSC Core will isolate peripheral blood mononuclear cells (PBMCs) from freshly collected whole blood. Cells will be cryopreserved at 5,000,000 cells per vial. The iPSC Core recommends collecting a minimum of two (2) 8ml CPT Vacutainer tubes. Please see the references below for more information on the collection of whole blood as well as the shipment of samples to the CBC or contact us.

Biobanking And PSC Thawing

As part of the custom iPSC generation service, the core will expand and bank three fully characterized iPSC clones per cell line. This service is also available to iPSC and ESC lines generated outside of Cedars-Sinai.

Service

Service Description

Requirements

Price/iPSC Line (1 clone) Academic

Price/iPSC Line (1 clone) Commercial

Cell Line Cryopreservation (5-6 vials)

Guaranteed cryopreservation of high quality, mycoplasma negative cells (6 vials total-feeder free).

Please provide the core with 1 well of starter cells.

$197

$334

PSC Thaw (1 week maintain)

The core will thaw 1 vial of PSCs into 1 Matrigel coated well of a 6-well plate and maintain cells for 1 week.

Please provide the core with 1 vial of cryopreserved cells.

$63

$111

PSC Thaw and Expansion (2 weeks)

The core will thaw 1 vial of PSCs into 1 Matrigel coated well of a 6-well plate and expand cells into a full 6-well plate over the course of 2 weeks.

Please provide the core with 1 vial of cryopreserved cells.

$212

$371

PSC Research Grade Biobank (20 vials)

The core will generate a research grade bank of multiple cryopreserved vials of PSCs.

Karyotype will be performed prior to initiating banking and at the time of creating a research grade lot.

Contact Us

Click here to contact the iPSC Core and Biomanufacturing Center.