Frequently Asked Questions
- Custom iPSC generation
- Banking and expansion of iPSC lines
- iPSC characterization
For more information on any of these services please visit Services.
The RMI iPSC Core can reprogram various human somatic tissues including fibroblast, lymphoblastoid cell lines, corneal epithelial cells, primary keratocytes, adipose stem cells, mammary epithelial cells and whole blood.
Currently the CBC iPSC Core cultures all iPSC and hESC lines by feeder-free method using BD Matrigel or Ultimatrix substrate and mTeSR Plus. Protocols for feeder-free culture can be found in Protocols.
Yes, visit the Cell Line page for a list of available cells lines and directions on how to order cells from the core. A Materials Transfer Agreement (MTA) must first be established between Cedars-Sinai and the requesting institution before any cells may be distributed. Each MTA specifies the requesting PI and is only valid for their specific investigation. If an MTA has already been established between institutions, and specifically for your project, an amendment to the original document is required to request additional lines.